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Buffered Charcoal Yeast Extract (BCYE) Feeley et al in 1978 developed a medium to isolate Legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract, and replacing starch with activated charcoal and named it as Charcoal Yeast Extract (CYE) Agar. Edelstein and Edelstein later compared agars used in the manufacture of buffered charcoal yeast extract medium and found significant differences in growth occurred. Oxoid Agar L11 gave the best results in the series tested 6. Characteristics of Legionella species ( Adapted from Renner & Tseng 10 ) Colonization of Francisella tularensis on various media, namely: (A) buffered charcoal yeast extract; (B) chocolate agar medium; (C) sheep’s blood agar; (D) cysteine heart agar. Pathogenicity: F. tularensis has two main pathogenic serotypes: Subspecies tularenis (type-A) and subspecies holarctica (type-B). Both types possess a thin BCYE Agar (Buffered Charcoal Yeast Extract), for Legionella, 15x100mm plate, order by the package of 10, by Hardy Diagnostics: Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria.

Edelstein and Edelstein later compared agars used in the manufacture of buffered charcoal yeast extract medium and found significant differences in growth occurred. Oxoid Agar L11 gave the best results in the series tested 6. Characteristics of Legionella species ( Adapted from Renner & Tseng 10 )

Abstract. Nocardia asteroides was isolated only from sputum samples, obtained from three patients with pulmonary nocardiosis, that had been cultured onto buffered charcoal-yeast extract (BCYE) and selective BCYE media as part of laboratory workups for Legionella species. BCYE (Buffered Charcoal Yeast Extract) Agar is used for the isolation and cultivation of Legionella species. II PERFORMANCE TEST PROCEDURE 1. Inoculate representative samples with the cultures listed below. a. Add 0.1 mL of a culture containing 30–300 CFU/0.1 mL to each plate and spread-inoculate using a sterile glass spreader. b.

Hardy Diagnostics Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria. W169 is based on Edelstein’s formula and is formulated in accordance with ISO 11371:(1998)E.

Yeast Extract provides sources of nitrogen, carbon, and vitamins in BCYE Agar. Activated Charcoal decomposes hydrogen peroxide and other toxic products. ACES Buffer was added to formulation to get a stable pH for optimal growth. α-Ketoglutarate stimulates organism growth. Agar is the solidifying agent. L-Cysteine, in the supplement, is an Allow sample to cool to room temperature before reading pH. Keep charcoal in suspension when dispensing. For solid medium, add 17 grams of Agar to base medium above. **Note: Medium should be wrapped in foil to protect from light. **Note: Be sure to allow solid products adequate time to dry (at least two days). Agar Hardy Diagnostics Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria. Do not ingest, inhale, or allow to come into contact with skin. This product is for in vitro diagnostic use only. In the experimental study, all 10 clinical isolates produced trails on buffered charcoal-yeast extract agar, and the mean recovery after 10 days of incubation ranged from 38% to 95% of the original inoculum number. For seven of the 10 isolates, more than 70% of the original inoculum was recovered on buffered charcoal-yeast extract agar. Thermo Scientific Remel Non-supplemented Buffered CYE Agar (Buffered charcoal yeast extract-BCYE) is used for the presumptive identification of Legionella spp. by satellite phenomena.